missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA
Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.
3400.00 SEK - 6690.00 SEK
Specifications
| Concentration | Endotoxin level: <0.1 EU/μg |
|---|---|
| Isolation Technology | Anion Exchange Chromatography Technology |
| Efficiency | Recovery of plasmid DNA: >90% |
| For Use With (Application) | High-copy plasmids from E. coli |
| Quantity | 1 Kit |
| Product Code | Brand | No. of Reactions | Yield | Sample Volume | Test Time | Price | Quantity & Availability | ||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Yield | Sample Volume | Test Time | Price | Quantity & Availability | ||
|
12863931
|
Macherey-Nagel™
740550 |
10 | 100–500μg | 30 - 150mL | 100 min./2 preps |
3400.00 SEK
10 reactions |
Please sign in to purchase this item. Need a web account? Register with us today! | ||
|
12860661
|
Macherey-Nagel™
740549 |
5 | 500–2,000μg | 150 - 500mL | 150 min./2 preps |
3695.00 SEK
5 reactions |
Please sign in to purchase this item. Need a web account? Register with us today! | ||
|
12873971
|
Macherey-Nagel™
740548 |
5 | 2,000–10,000μg | 500 - 2000mL | 180 min./2 preps |
6690.00 SEK
5 preps |
Please sign in to purchase this item. Need a web account? Register with us today! | ||
Description
An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.
Features
- Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
- Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
- NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
- Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
- AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours
Specifications
- DNA with less than 0.1 EU/μg DNA
- Purity of plasmid DNA: A260/A280 = 1.80–1.95
- Recovery of plasmid DNA >90%
- Structural integrity >90%
- Yield between 500μg up to 10mg, depending on kit used
Specifications
| Endotoxin level: <0.1 EU/μg | |
| Recovery of plasmid DNA: >90% | |
| 1 Kit | |
| Yes |
| Anion Exchange Chromatography Technology | |
| High-copy plasmids from E. coli | |
| A260/A280: 1.80–1.95 | |
| <300 kbp |
Safety and Handling
Hazard Category
- Flammable liquid Category 3
- Substances and mixtures corrosive to metals Category 1
- Skin corrosion/irritation Category 2
- Respiratory sensitiser Category 1
- Serious eye damage/eye irritation Category 2
- Skin sensitiser Category 1
Spot an opportunity for improvement?Share a Content Correction
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title