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Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA
Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.
Brand: Macherey-Nagel™ 740548
This item is not returnable.
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Description
An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.
Features
- Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
- Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
- NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
- Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
- AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours
Specifications
- DNA with less than 0.1 EU/μg DNA
- Purity of plasmid DNA: A260/A280 = 1.80–1.95
- Recovery of plasmid DNA >90%
- Structural integrity >90%
- Yield between 500μg up to 10mg, depending on kit used
Specifications
| Endotoxin level: <0.1 EU/μg | |
| Anion Exchange Chromatography Technology | |
| High-copy plasmids from E. coli | |
| 5 | |
| 1 Kit | |
| Yes | |
| <300 kbp | |
| 2,000–10,000μg |
| Gravity-flow Column (Giga) | |
| Recovery of plasmid DNA: >90% | |
| NucleoBond AX 10000 Columns, buffers, RNase A, NucleoBond Bottle Top Filters | |
| NucleoBond™ PC 10,000 EF | |
| A260/A280: 1.80–1.95 | |
| 500 - 2000mL | |
| 180 min./2 preps |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Flammable liquid Category 3
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
Hazard Statement
- H226-Flammable liquid and vapour.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
Supplemental information
- MIXTURE LIST-Contains : ethanol (diluted < 20 %)/sodium hydroxide solution (diluted< 2 %)/dodecyl sulfate, sodium salt
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