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  5. Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA
  6. 12860661

Macherey-Nagel™ NucleoBond™ PC EF, kit for endotoxin-free plasmid DNA

Removes endotoxins via an additional washing step, without ice incubation. Macherey-Nagel™ NucleoBond™ PC EF Kit is a classic anion exchanger for purification of endotoxin-free plasmid DNA. The kit includes NucleoBond™ AX Columns, all buffers, RNase A and NucleoBond™ Filters.

Brand:  Macherey-Nagel™ 740549


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Product Code. 12860661

  • 3695.00 SEK / 5 reactions

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Description

Description

An important prerequisite for transfections and gene therapy applications is endotoxin-free plasmid DNA. The patented procedure of NucleoBond™ PC EF reduces endotoxins to a very low level using the same working procedure as the standard protocol. In comparison to competitive kits, there is no need for a time-consuming incubation step to remove the endotoxins.

Features

  • Fast and easy clarification by NucleoBond™ Folded Filters—No centrifugation required, no shearing forces
  • Proven anion-exchange technology—Yields transfection-grade high-copy plasmid DNA from E. coli
  • NucleoBond™ Columns contain NucleoBond™ AX Silica Resin packed between inert filters
  • Polypropylene columns are resistant to organic solvents and are free of DNase and RNase
  • AX Resin can be used over a wide pH range and can remain in contact with buffers for up to three hours

Specifications

  • DNA with less than 0.1 EU/μg DNA
  • Purity of plasmid DNA: A260/A280 = 1.80–1.95
  • Recovery of plasmid DNA >90%
  • Structural integrity >90%
  • Yield between 500μg up to 10mg, depending on kit used
Specifications

Specifications

Endotoxin level: <0.1 EU/μg
Anion Exchange Chromatography Technology
High-copy plasmids from E. coli
5
1 Kit
Yes
<300 kbp
500–2,000μg
Mega Gravity-flow Column
Recovery of plasmid DNA: >90%
NucleoBond AX 2000 Columns, buffers, RNase A, NucleoBond Bottle Top Filters
NucleoBond™ PC 2,000 EF
A260/A280: 1.80–1.95
150 - 500mL
150 min./2 preps
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Hazard Category
  • Flammable liquid Category 3
  • Substances and mixtures corrosive to metals Category 1
  • Skin corrosion/irritation Category 2
  • Respiratory sensitiser Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin sensitiser Category 1

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