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Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit

Product Code. 10351214 Shop All Thermo Scientific Products
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Quantity:
25 reactions kit
Unit Size:
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Easily capture and affinity purify binding partners of His-tagged fusion proteins using nickel-chelate (Ni-IDA) agarose beads and this complete reagent set.

Thermo Scientific™ Pierce PolyHis Protein Interaction Pull-Down Kit purifies protein interaction complexes involving practically any user-supplied His-tagged fusion protein.

The PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, cobalt-IMAC affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Highlights:

  • 6xHis pull-down – purifies protein interactors of any His-tagged fusion protein

  • Complete kit – provides all components and detailed protocol for purifying protein:protein interactions

  • No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)

  • Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of cobalt IMAC agarose beads, including simple processing of multiple samples

  • Flexible – instructions include protocols for bait and prey proteins from different sources

Includes:

Cobalt chelate agarose resin, lysis buffer, TBS pack, imidazole solution for elution, microcentrifuge spin columns and collection tubes

Recommended for:

Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
• Cobalt Chelate Agarose Resin, 750 μL
• Pull-Down Lysis Buffer, 250 mL
• Imidazole Solution (4M), 5 mL
• TBS Pack (makes 500 mL), 1 pack
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 100 tubes

Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
Assay Affinity Purification
For Use With (Equipment) Microcentrifuge
Product Line Pierce™
Quantity 25 reactions kit
For Use With (Application) Pull-Down (Tag-Based)
Target Not Target-Specific
Type Pull-Down Kit
Sufficient For 25 Reactions
Format Kit

Safety and Handling

compliance-icons compliance-icons compliance-icons compliance-icons
Product Identifier
  • Pierce His Protein Interaction Pull-Down Kit
 Signal Word
  • Danger
 Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Reproductive toxicity Category 1B
  • Skin corrosion/irritation Category 1 B
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H360-May damage fertility or the unborn child.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P330-Rinse mouth.
  • P331-Do NOT induce vomiting.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P363-Wash contaminated clothing before reuse.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : 1-Imidazole, ethanol, Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.

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