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Gibco™ N-2 Supplement (100X)
Description
Gibco N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein's N-1 formulation, containing optimized concentrations of insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite. N-2 Supplement is recommended for growth and expression of neuroblastomas and neural cells in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).
N-2 Supplement has been demonstrated in wide-ranging applications, such as the culture of neural stem cells, the culture of the SH-SY5Y cell line (subline of the neuroblastoma cell line SK-N-SH), and culture of the HT22 mouse hippocampal neuronal cell line. Additionally, N-2 Supplement adds support to cell culture media used to differentiate pluripotent stem cells (PSCs) to a variety of cell types, including neural and pancreatic-like cell types.
N-2 Supplement may be used with Neurobasal media supplemented with growth factors such as bFGF and EGF. When combined with Dulbecco's Modified Eagle Medium (D-MEM) and fetal bovine serum (FBS), N-2 Supplement also provides suitable supplementation for the culture of human and rodent astrocytes.
- Each lot is cGMP manufactured
- Each lot is tested for sterility, endotoxin, and performance
- Contains insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite
- Can be used as a substitute for Bottenstein's N-1 formulation
- Provided as a 100X concentrate
Choose Gibco N-2 Supplement for a reliable, high-quality product trusted by researchers worldwide.
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -20°C) and protect from light. |
| Cell Type | Neural Cells |
| Classification | Serum-free |
| Concentration | 100 X |
| Culture Type | Mammalian Cell Culture |
| Form | Liquid |
| Product Type | Cell Culture Supplement |
| Serum Level | Serum-Free |
| Sterility | Sterile |
| pH | 6 to 8 |
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Safety and Handling
- Mercuric acetate
- Danger
- Acute toxicity Category 1
- Acute toxicity Category 2
- LONG-TERM AQUATIC HAZARD Chronic 1
- ACUTE AQUATIC Acute 1
- Specific target organ toxicity after repeated exposure Category 2
- H300-Fatal if swallowed.
- H310-Fatal in contact with skin.
- H330-Fatal if inhaled.
- H373-May cause damage to organs.
- H400-Very toxic to aquatic life.
- H410-Very toxic to aquatic life with long lasting effects.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P310-Immediately call a POISON CENTER/doctor/.
- P362-Take off contaminated clothing.
- MIXTURE LIST-Contains : Mercuric acetate
For Research Use Only. Not for use in diagnostic procedures.
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