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Invitrogen™ Mouse IL-18 ELISA Kit

ELISA

6430.00 SEK - 34290.00 SEK

Specifications

Accession Number P70380
Assay Range 31.3 to 2,000 pg/mL
Assay Sensitivity 19.0 pg/mL
Conjugate HRP
Product Type ELISA
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-18
Biotin-Conjugate anti-mouse IL-18 monoclonal antibody
Streptavidin-HRP
Mouse IL-18 Standard lyophilized, 4ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

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Pack of 10
Estimated Shipment: 25-03-2025
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Description

Description

The Mouse Interleukin-18 (Ms IL-18) ELISA quantitates Ms IL-18 in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-18. Principle of the method The Mouse IL-18 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Specifications

Specifications

P70380
19.0 pg/mL
ELISA
Mouse
Colorimetric Microplate Reader
Igif,Il-18
2.2
HRP
Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Interleukin-18, Interferon-gamma-inducing factor
1 hrs 20 mins
31.3 to 2,000 pg/mL
HRP
Plasma, Serum, Supernatant
ELISA
16173
2.2%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
IL-18
3 hrs 10 mins
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