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Invitrogen™ Fluo-5N, Pentapotassium Salt, cell impermeant

Analog of fluo-4 with lower Ca2+-binding affinity, making it suitable for detecting intracellular calcium levels in 1μM to 1mM range that would saturate response of fluo-3 and fluo-4

Brand:  Invitrogen™ F14203

Product Code. 11514786

  • 3985.00 SEK / 500µg

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca2+.

  • Cells may be physically loaded with cell-impermeant salt forms of these indicators using patch pipet or microinjection or Influx™ pinocytotic cell-loading reagent
  • Compatible with excitation at 488nm by argon-ion laser sources, making them useful for confocal microscopy, flow cytometry, and microplate screening applications
  • Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
  • BAPTA-based indicators bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
  • Perturbations to calcium measurements caused by presence of the ions can be controlled using heavy metal-selective chelator

  • Label (Ex/Em of Ca2+-bound form): Fluo-5N (494/516nm)
  • Fluorescence intensity increase upon binding Ca2+: >100 fold
  • Kd for Ca2+ in buffer: approximately 90μM

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Store in freezer -5°C to -30°C and protect from light.
Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Fluorescent Dye-Based
Room Temperature
Fluorescence
Calcium Indicator
500 μg
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For Research Use Only. Not for use in diagnostic procedures.