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Thermo Scientific™ Mem-PER™ Eukaryotic Membrane Protein Extraction Kit

Product Code. 10658694 Shop All Thermo Scientific Products
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Quantity:
75 mL Kit
Unit Size:
1 set
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Perform small-scale hydrophobic membrane-associated protein solubilization and enrichment with this simple reagent-based procedure.

Thermo Scientific™ Mem-PER Eukaryotic Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of hydrophobic membrane-associated proteins in a simple reagent-based procedure.

Traditional methods for isolation of membrane protein are tedious and time-consuming, and they require gradient separation and expensive ultracentrifugation equipment. The Mem-PER Kit effectively isolates membrane proteins from cultured cells and tissues using a simple benchtop microcentrifuge procedure. Hydrophobic proteins having three or fewer membrane-spanning domains are effectively solubilized and isolated from hydrophilic (cytoplasmic) proteins using the three-reagent system. The kit makes possible the extraction and selective enrichment of integral and attached membrane proteins from cultured mammalian and yeast cells, as well as from hard and soft mammalian tissues.

Highlights:

  • Extraction and isolation – produces minimal cross-contamination (typically <10%) of hydrophilic protein into the hydrophobic (membrane) protein fraction

  • Cells or tissues – extracts protein from cultured mammalian and yeast cells and mammalian tissues

  • Downstream compatibility – analyze membrane protein extracts by SDS-PAGE, Western blotting and protein assays

  • Fast and simple – isolate membrane proteins in approximately one hour

  • No special equipment required – requires only a benchtop microcentrifuge, tubes and pipetters

Includes:

Three buffers and detergent solutions necessary to make working reagents

TRUSTED_SUSTAINABILITY

Specifications

Formulation Three-component kit
Description Mem-PER Eukaryotic Membrane Protein Extraction Kit
Includes Mem-PER Reagent C, 40mL; Mem-PER Reagent B, 25mL; Mem-PER Reagent A, 10mL
Target Proteins Membrane
Donor Source Cultured Mammalian Cells, Cultured Yeast Cells, Animal Tissue
Sufficient For 60 to 80 samples each containing 5 million cells, 15mg yeast cell paste or 20mg tissue
Quantity 75 mL Kit
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