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  5. IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
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Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

Brand:  IBA Lifesciences 5-5043-001

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Product Code. 15673858

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Description

Description

pCSG-IBA mammalian expression vectors

  • Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
  • Stable cell lines can be selected by the neomycin resistance gene (NeoR).
  • In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Specifications

Specifications

Constitutive
CMV
Geneticin™ (G-418)
Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Room temperature
Direct Cloning
Forward
pCSG-IBA43
Ampicillin
Vector
12 months after shipping
Mammalian
His Tag (6x), Strep-tag II
250 ng/μL
5 μg
Episomal expression (oriP, EBNA-1)
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