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Invitrogen™ AccuPrime™ Pfx SuperMix

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

Brand: Invitrogen™ 12344040

4240.21 SEK valid until 2025-03-21
Use promo code "24111" to get your promotional price.

Product Code. 10043322

6360.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

Alert:

To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price

Includes: AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO₄ (pH 8.4), 30.8mM (NH₄)₂SO₄, 11mM KCl, 1.1mM MgSO₄, 330μM dNTPs, AccuPrime proteins, stabilizers.

Requires : Template and primers
This item is not returnable. View return policy

Description

Invitrogen AccuPrime Pfx SuperMix provides reagents for high-fidelity, high-specificity amplification of DNA fragments. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. This ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs is designed for efficient PCR amplification.

Features

>26-times higher fidelity than with Taq DNA polymerase
Reduced set-up time
Produces products with blunt ends

Applications

High-fidelity PCR
Amplification of fragments up to 15 kb
Cloning & mutagenesis

Notes

Thermostable AccuPrime Pfx SuperMix proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Content And Storage	AccuPrime Pfx SuperMix (4 × 1.125 mL) Store at –20°C in a non-frost-free freezer.
GC-Rich PCR Performance	Low
Reaction Speed	Standard
Shipping Condition	Wet or Dry Ice
Concentration	1.1X
Hot Start	Built-In Hot Start
Overhang	Blunt
Size (Final Product)	15 kb or less

Detection Method	Primer-probe
Polymerase	AccuPrime Pfx DNA Polymerase
Quantity	200 rxns
For Use With (Application)	Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq)	26X
No. of Reactions	200 Reactions
Reaction Format	SuperMix or Master Mix

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For Research Use Only. Not for use in diagnostic procedures.

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