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Cytiva RNAspin Mini Isolation Kit
Extract high-quality RNA from a wide range of samples rapidly. Cytiva Life Sciences™illustra™ RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria, or yeast.
Brand: Cytiva
Includes: Protocol booklet with detachable, quick reference card, necessary components for specified number of preps, including prefilters and DNase I
Description
- Recovers total RNA due to removal of genomic DNA through on-column DNase I treatment
- Can be used on cultured cells, tissue, yeast and hard-to-lyse bacterial strains
- Prefilters reduce lysate viscosity for maximum yield and purity
- Results can be obtained with small amounts of sample
- Binding capacity: 100μg
- Sample size: 30mg (animal tissue), up to 5 × 106 cells (cell culture)
- Elution volume: 40 to 120μL
- RNA Purity (A260/A280): 1.8 to 2.2
- Total time of preparation: <30 min/6 preps
Quantitative reverse transcriptase PCR (QRT-PCR), Northern blot, and microarray experiments
Specifications
| illustra RNAspin Mini RNA Isolation Kit is designed for rapid extraction of high-quality RNA from a wide range of sample types. | |
| Protocol booklet with detachable, quick-reference guide and necessary components, including prefilters and DNase I | |
| illustra RNAspin Mini Kit | |
| <30 mg | |
| Up to 100μg |
| Used for the isolation of high-quality total RNA from a wide range of source material including hard-to-lyse bacterial strains. Purified RNA is suitable for use in sensitive downstream applications such as quantitative reverse transcriptase PCR (qRT-PCR), | |
| 250 Preps | |
| 1.8-2.2 | |
| < 30 min/6 preps |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Flammable liquid Category 3
- Respiratory sensitiser Category 1
- Skin sensitiser Category 1
Hazard Statement
- H226-Flammable liquid and vapour.
- H412-Harmful to aquatic life with long lasting effects.
- H312-Harmful in contact with skin.
- H317-May cause an allergic skin reaction.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H302-Harmful if swallowed.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P273-Avoid release to the environment.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol, thiocyanide, deoxyribose nuclease
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