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  5. Cytiva HiTrap™ rProtein A FF Columns

Cytiva HiTrap™ rProtein A FF Columns

Product Code. 10724547 Shop All Cytiva Products
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Quantity:
1 x 5 mL
2 x 1 mL
5 x 1 mL
5 x 5 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
Unit Size:
Each
Pack of 2
Pack of 5
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Use for preparative purification of monoclonal and polyclonal antibodies from cell culture supernatants, serum and ascites. Cytiva Life Sciences™ HiTrap™ rProtein A FF Columns are prepacked with rProtein A Sepharose™ Fast Flow.

  • Packed with rProtein A Sepharose™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources
  • Exhibits similar Fc region specificity to that of native protein A but with enhanced binding capacity and fewer regulatory concerns due to total absence of mammalian culture in ligand production and purification
  • Average particle size: 90μm
  • Designed for use with a syringe, a peristaltic pump, or a chromatography system
  • Available as 1mL and 5mL columns
Media
  • Ligand: Recombinant protein A (E.coli)
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: ∽50mg human IgG/mL
  • pH stability working range: 3 to 10

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.
5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Order Info

1mL and 5mL columns are packed with 1mL and 5mL of rProtein A Sepharose Fast Flow, respectively.

TRUSTED_SUSTAINABILITY

Specifications

Binding Capacity ≈50 mg Human IgG/mL
Flow Rate <20 mL/min.
For Use With (Application) Chromatography System, Manual
For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
Content And Storage 4°C to 30°C, 20% Ethanol
Quantity 1 x 5 mL

Safety and Handling

compliance-icons
Product Identifier
  • HiTrap rProtein A FF 5 ml
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : Ethanol
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