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Cytiva GSTPrep™ FF 16/10 Column
Fast and easy one-step, scale-up purification of GST-tagged recombinant proteins
Brand: Cytiva 28-9365-50
This item is not returnable.
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Description
Glutathione Sepharose™ 4 Fast Flow is prepacked in 20mL GSTPrep columns for purification of GST-tagged proteins (as well as glutathione S-transferases and glutathione-dependent proteins). Mild elution conditions preserve protein antigenicity and function.
Features:
- Binds approx. 200mg recombinant GST/column
- Simple operation with pump or chromatography system (e.g., AKTA™)
GSTPrep FF 16/10 Prepacked Columns
- Volume: 20mL
- Dimensions: 1.6 x 10cm
- Recommended flowrate (H2O at room temp): 1 to 10mL/min. (30 to 300cm/hr.)
- Max. flowrate (H2O at room temp): 10mL/min. (300cm/hr.)
- Max. pressure during operation (H2O at room temp): 1.5bar (0.15MPa, 22psi)
- Hardware pressure limit: 5bar (0.5MPa, 73psi)
- Storage/temperature: 20% ethanol/4° to 30°C
Specifications
| 1M Acetate, pH 6, 0.1M NaOH, 70% Ethanol or 6M Guanidine Hydrochloride | |
| 20mL | |
| 16mm | |
| 3.9 in. | |
| <10mL/min. | |
| 1.5 bar | |
| 90μm | |
| 4° to 30°C | |
| Purification of Glutathione S-transferases and Glutathione-dependent Proteins |
| GSTPrep Glutathione Sepharose 4 FF column | |
| 0.62 in. | |
| 1 x 20 mL | |
| 100mm | |
| Glutathione and 10 Carbon linker arm | |
| 3 to 12 | |
| 4% Highly Cross-linked Agarose | |
| Ion Exchange Column |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- GSTPrep FF 16/10
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol
- UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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