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Cytiva Thrombin Protease

Product Code. 10218854 Shop All Cytiva Products
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500 units
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

  • Supplied as lyophilized powder
  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

TRUSTED_SUSTAINABILITY

Specifications

Buffer Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4 pH 7.3
Product Type Thrombin Protease Fusion Protein
Quantity 500U
For Use With (Application) Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours

Safety and Handling

compliance-icons compliance-icons
Signal Word
  • Danger
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : thrombin
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