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Gibco™ B-27™ Supplement (50X), minus vitamin A
Description
Gibco B-27 Supplement minus vitamin A is a customized, serum-free formulation based on the original, most cited neuronal cell culture supplement, designed specifically to support the proliferation and cultivation of neural stem cells (NSCs) and neural progenitor cells. By excluding vitamin A (also known as retinyl acetate or retinol), this variant prevents the differentiation-inducing effects of retinoic acid, making it ideal for maintaining NSCs and neural progenitors in their undifferentiated state.
- Optimized for NSC proliferation—by removing vitamin A, this supplement is perfectly suited for the growth and expansion of embryonic and adult neural stem cells and neural precursors, without inducing differentiation
- Versatile cultivation—supports the cultivation of neural progenitor cells and NSCs in both neurospheres in suspension and adherent monolayer cultures, providing flexibility in your experimental design. Ideal for cell culture where retinoic acid signaling must be controlled.
- High quality—cGMP-manufactured to ensure the highest standards of quality and consistency. Each batch undergoes rigorous performance testing, including assessments of its ability to support the differentiation of human neural stem cells to neurons.
- Comprehensive testing—every lot is tested for endotoxins, sterility, and mycoplasma to ensure the utmost safety and reliability
- Proven performance—derived from the proprietary formulation of the original B-27 Supplement, trusted by researchers worldwide for its consistent and reliable results
Choose Gibco B-27 Supplement, minus vitamin A, for an optimized, high-quality solution that supports the proliferation of neural stem cells while preventing unwanted differentiation influenced by retinoic acid, ensuring consistent and reliable results for your research.

Specifications
Specifications
Content And Storage | Store in freezer (-5 to -20°C) and protect from light. |
Cell Type | Neural Progenitor Cells |
Classification | Serum-free |
Concentration | 50 X |
Culture Type | Mammalian Cell Culture |
Form | Liquid |
Product Type | Cell Culture Supplement |
Serum Level | Serum-Free |
Sterility | Sterile |
Product Line | B-27™ |
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Safety and Handling

- 5-Methoxyindole
- Warning
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- MIXTURE LIST-Contains : Indol-5-yl methyl ether
For Research Use Only. Not for use in diagnostic procedures.
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